DNA Microarray-Based PCR Ribotyping of Clostridium difficile
نویسندگان
چکیده
منابع مشابه
Molecular Analysis of Toxigenic Clostridium difficile Isolates from Hospital Environment by PCR Ribotyping Method
Background and Aims: Clostridium difficile is an identified cause of antibiotic-associated diarrhea, antibiotic-associated colitis, pseudomembranous colitis and nosocomial diarrhea. The objective of this survey was to determine molecular analysis of toxigenic Clostridium difficile isolates from hospital environment in Tehran tertiary medical centers. Materials and Methods: In this descriptiv...
متن کاملCharacterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping
We have developed a Clostridium difficile PCR ribotyping method based on capillary gel electrophoresis and have compared it with conventional PCR ribotyping. A total of 146 C. difficile isolates were studied: five isolates were reference strains (PCR ribotypes 001, 014, 017, 027 and 053); 141 were clinical isolates comprising 39 Austrian PCR ribotypes collected in the period 2006-2007 at 25 Aus...
متن کاملComparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.
Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. However, the comparative typing ability, reproducibility, discriminatory power, ...
متن کاملEvaluation of portability and cost of a fluorescent PCR ribotyping protocol for Clostridium difficile epidemiology.
Clostridium difficile is the most commonly identified pathogen among health care-associated infections in the United States. There is a need for accurate and low-cost typing tools that produce comparable data across studies (i.e., portable data) to help characterize isolates during epidemiologic investigations of C. difficile outbreaks and sporadic cases of disease. The most popular C. difficil...
متن کاملDevelopment and Validation of an Internationally-Standardized, High-Resolution Capillary Gel-Based Electrophoresis PCR-Ribotyping Protocol for Clostridium difficile
PCR-ribotyping has been adopted in many laboratories as the method of choice for C. difficile typing and surveillance. However, issues with the conventional agarose gel-based technique, including inter-laboratory variation and interpretation of banding patterns have impeded progress. The method has recently been adapted to incorporate high-resolution capillary gel-based electrophoresis (CE-ribo...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2014
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.02524-14